Research areas
has focussed upon protein biogenesis within a group of positive-stranded RNA viruses, the Picornaviruses. Such viruses possess a single, long, open reading frame, but must generate multiple proteins for their RNA replication and encapsidation: they encode a 'polyprotein'. My work at the IAH, Pirbright on Foot-and-Mouth Disease virus (FMDV) indicated that one polyprotein 'cleavage' event was most unusual. This work was carried forward and expanded at St 糖心logo where we determined that this 'cleavage' event was not proteolytic, but was a novel translational 'recoding' event mediated by an oligopeptide sequence: '2A'. Our present work involves the molecular mechanism of this recoding event, the use of this system for biomedical and biotechnological applications and the occurrence of '2A-like sequences in other viral and cellular sequences.
Relatively recently, permission was granted by HSE/DEFRA for St 糖心logo to work with the FMDV 'replicon' - stimulating a major new line of research. In collaboration with the Pibright Institute and the Universities of Leeds, Dundee and Edinburgh (initially funded by the Wellcome Trust, but now funded by a BBSRC SLoLa grant), the consortium has embarked upon a comprehensive study of the molecular biology of FMDV replication. A substantial component of this program of work involves harnessing modern molecular approaches for the development of a new generation of FMDV vaccines.
Selected publications
-
Open access
Luke, G. A., Ross, L. S., Lo, Y.-T., Wu, H.-C. & Ryan, M. D., 9 Oct 2024, In: Viruses. 16, 10, 17 p., 1587.
Research output: Contribution to journal 鈥� Article 鈥� peer-review
-
Open access
Lo, Y. T., Ryan, M. D., Luke, G. A., Chang, W. C. & Wu, H. C., 6 Jan 2023, In: Scientific Reports. 13, 11 p., 296.
Research output: Contribution to journal 鈥� Article 鈥� peer-review
-
Open access
Lo, Y.-T., Tulloch, F., Wu, H.-C., Luke, G. A., Ryan, M. D. & Chu, C.-Y., Sept 2021, In: Journal of Applied Microbiology. 131, 3, p. 1123-1135 12 p.
Research output: Contribution to journal 鈥� Article 鈥� peer-review
-
Open access
Nour, I., Hanif, A., Ryan, M. D. & Eifan, S., 8 Oct 2021, In: Water. 13, 19, 34 p., 2794.
Research output: Contribution to journal 鈥� Article 鈥� peer-review
-
Open access
Ryan, M. D. & Luke, G. A., 14 May 2021, In: Journal of Infectious Diseases & Preventive Medicine. 9, 4, p. 1-3 3 p., 1000223.
Research output: Contribution to journal 鈥� Review article 鈥� peer-review
-
Open access
Lasecka-Dykes, L., Tulloch, F., Simmonds, P., Luke, G. A., Ribeca, P., Gold, S., Knowles, N. J., Wright, C. F., Wadsworth, J., Azhar, M., King, D. P., Tuthill, T. J., Jackson, T. & Ryan, M. D., 25 Aug 2021, In: mSphere. 6, 4, p. 1-19 19 p., e00015-21.
Research output: Contribution to journal 鈥� Article 鈥� peer-review
-
Ryan, M. D. & Luke, G. A., 1 Mar 2021, Encyclopedia of virology: Volume 1 The virus as a concept - Fundamentals of virology. Bamford, D. H. & Zuckerman, M. (eds.). 4th ed. New York: Academic Press/Elsevier , p. 444-459 16 p.
Research output: Chapter in Book/Report/Conference proceeding 鈥� Chapter
-
Grabert, K., Sehgal, A., Irvine, K. M., Wollscheid-Lengeling, E., Ozdemir, D. D., Stables, J., Luke, G. A., Ryan, M. D., Adamson, A., Humphreys, N. E., Sandrock, C. J., Rojo, R., Verkasalo, V. A., Mueller, W., Hohenstein, P., Pettit, A. R., Pridans, C. & Hume, D. A., 1 Dec 2020, In: The Journal of Immunology. 205, 11
Research output: Contribution to journal 鈥� Article 鈥� peer-review
-
Open access
Tulloch, F., Luke, G. A. & Ryan, M. D., 5 Jul 2018, In: Journal of Veterinary Medicine and Research. 5, 5, 6 p., 1140.
Research output: Contribution to journal 鈥� Review article 鈥� peer-review
-
Open access
Luke, G. A. & Ryan, M. D., Nov 2018, In: Reviews in Medical Virology. 28, 6, 12 p., e2001.
Research output: Contribution to journal 鈥� Review article 鈥� peer-review